blotting roller aluminum foil imaging system Search Results


sapphire biomolecular imager  (Azure Biosystems)
97
Azure Biosystems sapphire biomolecular imager
Sapphire Biomolecular Imager, supplied by Azure Biosystems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/Sapphire+Biomolecular+Imager/bio_rxiv__2024__09__30__615654-263-6-9
Average 97 stars, based on 1 article reviews
sapphire biomolecular imager - by Bioz Stars, 2026-10
97/100 stars
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sapphire fl biomolecular imager  (Azure Biosystems)
95
Azure Biosystems sapphire fl biomolecular imager
Sapphire Fl Biomolecular Imager, supplied by Azure Biosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/SAPPHIRE+FL+BIOMOLECULAR+IMAGER/bio_rxiv__64898__2026__02__07__704091-429-10-14
Average 95 stars, based on 1 article reviews
sapphire fl biomolecular imager - by Bioz Stars, 2026-10
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falcon tubes  (Bio-Rad)
92
Bio-Rad falcon tubes
Falcon Tubes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/Tube+Roller/pm41854129-333-159-175
Average 92 stars, based on 1 article reviews
falcon tubes - by Bioz Stars, 2026-10
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carboxyphenol ba  (Chem Impex International)
95
Chem Impex International carboxyphenol ba
Carboxyphenol Ba, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/Salicylic+acid/pm33543929-33-0-5
Average 95 stars, based on 1 article reviews
carboxyphenol ba - by Bioz Stars, 2026-10
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gel blot roller  (Bio-Rad)
93
Bio-Rad gel blot roller
Gel Blot Roller, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/Roller/pm41854129-333-144-146
Average 93 stars, based on 1 article reviews
gel blot roller - by Bioz Stars, 2026-10
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gene exp acp5 mm00475698 m1  (Thermo Fisher)
99
Thermo Fisher gene exp acp5 mm00475698 m1
Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , <t>acp5</t> , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.
Gene Exp Acp5 Mm00475698 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/Gene+Exp%2E+Acp5%2C+Mm00475698_m1/pmc12840716-214-11--1
Average 99 stars, based on 1 article reviews
gene exp acp5 mm00475698 m1 - by Bioz Stars, 2026-10
99/100 stars
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imagn imaging cytometer  (Becton Dickinson)
90
Becton Dickinson imagn imaging cytometer
Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , <t>acp5</t> , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.
Imagn Imaging Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/imagn+imaging+cytometer/pm21538841-23-17-20
Average 90 stars, based on 1 article reviews
imagn imaging cytometer - by Bioz Stars, 2026-10
90/100 stars
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kiestra™ imaga bt digital imaging module  (Becton Dickinson)
90
Becton Dickinson kiestra™ imaga bt digital imaging module
Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , <t>acp5</t> , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.
Kiestra™ Imaga Bt Digital Imaging Module, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/kiestra++imaga+bt+digital+imaging+module/pmc06138813-67-9-8
Average 90 stars, based on 1 article reviews
kiestra™ imaga bt digital imaging module - by Bioz Stars, 2026-10
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tpitm imaga 1000 imaging system  (Teraview Ltd)
90
Teraview Ltd tpitm imaga 1000 imaging system
Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , <t>acp5</t> , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.
Tpitm Imaga 1000 Imaging System, supplied by Teraview Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/tpitm+imaga+1000+imaging+system/us08023110-102-11-17
Average 90 stars, based on 1 article reviews
tpitm imaga 1000 imaging system - by Bioz Stars, 2026-10
90/100 stars
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imago imaging system  (Isogen Life Science)
90
Isogen Life Science imago imaging system
Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , <t>acp5</t> , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.
Imago Imaging System, supplied by Isogen Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/imago+imaging+system/pmc02440944-99-16-19
Average 90 stars, based on 1 article reviews
imago imaging system - by Bioz Stars, 2026-10
90/100 stars
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imaga bt digital imaging module  (Becton Dickinson)
90
Becton Dickinson imaga bt digital imaging module
Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , <t>acp5</t> , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.
Imaga Bt Digital Imaging Module, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/imaga+bt+digital+imaging+module/10__1515_slash_cclm___2016___0370-717-7-12
Average 90 stars, based on 1 article reviews
imaga bt digital imaging module - by Bioz Stars, 2026-10
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thz pulsed imaging system imaga 2000  (Teraview Ltd)
90
Teraview Ltd thz pulsed imaging system imaga 2000
Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , <t>acp5</t> , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.
Thz Pulsed Imaging System Imaga 2000, supplied by Teraview Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/thz+pulsed+imaging+system+imaga+2000/10__1021_slash_acsphotonics__5b00672-73-8-15
Average 90 stars, based on 1 article reviews
thz pulsed imaging system imaga 2000 - by Bioz Stars, 2026-10
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Image Search Results


Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , acp5 , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.

Journal: International Journal of Molecular Sciences

Article Title: Porphyromonas gingivalis Vesicles Control Osteoclast–Macrophage Lineage Fate

doi: 10.3390/ijms27020831

Figure Lengend Snippet: Stage-dependent effects of Pg -OMVs on OC-genesis. ( A ) Schematic of three stimulation schedules used in RAW264.7 cultures: Schedule 1 (S1), simultaneous stimulation with RANKL and Pg -OMVs at Day 0; Schedule 2 (S2), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 1; and Schedule 3 (S3), RANKL priming at Day 0 followed by Pg -OMVs stimulation at Day 3. In all protocols, cultures were maintained for 7 days, and TRAP staining, pit-formation assays, mRNA/protein expression analyses, and cytokine analyses were performed. ( B ) Representative TRAP staining and hydroxyapatite pit resorption images under each stimulation schedule. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( C ) Quantification of TRAP-positive multinucleated cells (>3 nuclei and >10 nuclei). ( D ) Quantification of pit resorption area. ( E ) Relative mRNA expression of osteoclast-related markers ( dcstamp , ocstamp , nfatc1 , acp5 , mmp9 ). ( F – H ) Western blot analyses of NFATc1 and OC-STAMP proteins normalized to GAPDH, with densitometric quantification. Results demonstrate that simultaneous OMV + RANKL stimulation (S1) suppressed osteoclast formation, whereas delayed Pg -OMVs addition (S2, S3) enhanced OC-genesis and upregulated OC-STAMP expression. The images were obtained from using the Azure C400. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. #: p < 0.05, vs. control. *: p < 0.05, vs. +RANKL+ Pg -OMVs, **: p ≤ 0.01, vs. +RANKL+ Pg -OMVs.

Article Snippet: The gene expressions of ocstamp (Mm00512445_m1), dcstamp (Mm04209236_m1), nfatc1 (Mm01265944_m1), acp5 (Mm00475698_m1), and mmp9 (Mm00442991_m1) were examined.

Techniques: Staining, Expressing, Imaging, Western Blot, Control

OC-STAMP mediates Pg -OMV-induced osteoclast fusion. ( A ) TRAP staining of RAW264.7 cells stimulated under S3 with or without neutralizing anti-OC-STAMP monoclonal antibody (xOC-STAMP mAb) or isotype IgG control. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( B ) Quantification of TRAP-positive multinucleated osteoclasts (>3 and >10 nuclei). ( C ) Expression of osteoclast-related genes ( dcstamp , nfatc1 , acp5 , mmp9 ) by qRT-PCR. Neutralization of OC-STAMP significantly reduced Pg -OMV-induced osteoclast fusion and gene expression, demonstrating the role of OC-STAMP in Pg -OMV-mediated OC-genesis. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. *: p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Porphyromonas gingivalis Vesicles Control Osteoclast–Macrophage Lineage Fate

doi: 10.3390/ijms27020831

Figure Lengend Snippet: OC-STAMP mediates Pg -OMV-induced osteoclast fusion. ( A ) TRAP staining of RAW264.7 cells stimulated under S3 with or without neutralizing anti-OC-STAMP monoclonal antibody (xOC-STAMP mAb) or isotype IgG control. Images of the samples were obtained using an EVOS™ XL Core Imaging System. Scale bars indicate 100 µm. ( B ) Quantification of TRAP-positive multinucleated osteoclasts (>3 and >10 nuclei). ( C ) Expression of osteoclast-related genes ( dcstamp , nfatc1 , acp5 , mmp9 ) by qRT-PCR. Neutralization of OC-STAMP significantly reduced Pg -OMV-induced osteoclast fusion and gene expression, demonstrating the role of OC-STAMP in Pg -OMV-mediated OC-genesis. Results were presented as the means ± SD (n = 3). Experiments were independently repeated three times with similar results. Statistical analyses were performed using the Kruskal–Wallis test followed by Steel–Dwass’s post hoc test for multiple comparisons. *: p < 0.05.

Article Snippet: The gene expressions of ocstamp (Mm00512445_m1), dcstamp (Mm04209236_m1), nfatc1 (Mm01265944_m1), acp5 (Mm00475698_m1), and mmp9 (Mm00442991_m1) were examined.

Techniques: Staining, Control, Imaging, Expressing, Quantitative RT-PCR, Neutralization, Gene Expression